How to Make an SDS-PAGE Gel: Step-by-Step Guide
How to Make an SDS-PAGE Gel: Step-by-Step Guide SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a cornerstone technique in molecular biology and biochemistry for separating proteins based on their size. In this post, we’ll walk you through how to prepare an SDS gel , including tips for separation and stacking gels , using a typical 10% SDS-PAGE gel recipe . Whether you’re a beginner or an experienced researcher, this step-by-step guide will help you make reliable gels for your experiments. Safety notes: Acrylamide is neurotoxic → always wear gloves & goggles. Use fresh APS (unstable in solution). Add TEMED last → starts polymerization immediately. Resolving Gel (for 100 ml, ×10) Reagent 7 % 8 % 10 % 12 % 15 % H₂O 3.8 ml 3.4 ml 2.8 ml 2.1 ml 1.1 ml 30 % Acrylamide 2.3 ml 2.7 ml 3....