Stripping of Westernblot membranes
Stripping of WB membranes protocol - Prewarm stripping box for 10 min. in 55 °C water bath with 100 mL stripping buffer + 680 µ L β -Mercaptoethanol (alternatively you can use 50 ml with 340 µl) - Put membrane in stripping buffer box in water bath for 20 minutes, after incubation pour stripping buffer in β -Mercaptoethanol waste - Wash 1x with distilled water; add water and let it incubate without shaking to evaporate β -Mercaptoethanol - Transfer membranes into new box with TBST buffer - Wash on shaker for 10 minutes 3x with TBST buffer - Prepare next antibody - Put in tubes with new antibody (no extra b...