Stripping of Westernblot membranes

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 Stripping of WB membranes protocol



-        Prewarm stripping box for 10 min. in 55 °C water bath with 100 mL stripping buffer + 680 µL β-Mercaptoethanol (alternatively you can use 50 ml with 340 µl)


-        Put membrane in stripping buffer box in water bath for 20 minutes, after incubation pour stripping buffer in β-Mercaptoethanol waste


-        Wash 1x with distilled water; add water and let it incubate without shaking to evaporate β-Mercaptoethanol


-        Transfer membranes into new box with TBST buffer


-        Wash on shaker for 10 minutes 3x with TBST buffer 


-        Prepare next antibody 


-        Put in tubes with new antibody (no extra blocking of membrane required) 


-        Incubate on shaker at 4 °C (cold room) over night 

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