Lab technician

 How to use a drigalski spatula  Prepare a 96% Ethanol solution   Place the drigalski spatula in the ethanol  Flame the spatula and let the ethanol burn (to sterilize)  Place the spatula on the agar plate and close the plate with the lid (to cool spatula) Add your bacteria  Disperse the bacteria with the spatula until the agar is dry  Place the plate with bacteria in the incubator at a desired temperature  Place the spatula back in ethanol and store at RT

 How to make a bacterial liquid culture  Preheat incubator to desired temperatur  Preheat media to desired temperatur Pipetet desired volume of medium into erlenmeyer flask   Add antibiotic if necessary  Pick a single colony with a pipette tip and drop it into the flask  Move flask to incubator  Incubate over night or for a certain time period  Proceed to use bacteria for downstreamm application